]> Finds restriction enzymes that produce a specific overhang (EMBOSS) nucleic:restriction http://bioweb.pasteur.fr/docs/EMBOSS/restover.html restover &emboss_init; input Input section sequence sequence -- DNA [sequences] (-sequence) perl " -sequence=$value -sformat=fasta" 1 dna 8 seqsfile perl 1 required Required section seqcomp Overlap sequence (-seqcomp) perl " -seqcomp=$value" 2 advanced Advanced section min Minimum cuts per RE (-min) 1 perl (defined $value && $value != $vdef)? " -min=$value" : "" 3 1 1000 max Maximum cuts per RE (-max) 2000000000 perl (defined $value && $value != $vdef)? " -max=$value" : "" 4 acd $min 2000000000 single Force single site only cuts (-single) 0 perl ($value)? " -single" : "" 5 threeprime 3' overhang? (else 5') e.g. BamHI has CTAG as a 5' overhang, and ApaI has CCGG as 3' overhang. (-threeprime) 0 perl ($value)? " -threeprime" : "" 6 blunt Allow blunt end cutters (-blunt) 1 perl ($value)? "" : " -noblunt" 7 sticky Allow sticky end cutters (-sticky) 1 perl ($value)? "" : " -nosticky" 8 ambiguity Allow ambiguous matches (-ambiguity) 1 perl ($value)? "" : " -noambiguity" 9 plasmid Allow circular DNA (-plasmid) 0 perl ($value)? " -plasmid" : "" 10 commercial Only enzymes with suppliers (-commercial) 1 perl ($value)? "" : " -nocommercial" 11 datafile Alternative RE data file (-datafile) perl ($value)? " -datafile=$value" : "" 12 output Output section html Create HTML output (-html) 0 perl ($value)? " -html" : "" 13 limit Limits reports to one isoschizomer (-limit) 1 perl ($value)? "" : " -nolimit" 14 alphabetic Sort output alphabetically (-alphabetic) 0 perl ($value)? " -alphabetic" : "" 15 fragments Show fragment lengths (-fragments) 0 perl ($value)? " -fragments" : "" 16 name Show sequence name (-name) 0 perl ($value)? " -name" : "" 17 outfile outfile (-outfile) outfile.out perl " -outfile=$value" 18 auto perl " -auto -stdout" 19