]> Selects primers for PCR and DNA amplification. (EMBOSS) nucleic:primers http://bioweb.pasteur.fr/docs/EMBOSS/prima.html prima &emboss_init; input Input section sequence sequence -- dna [single sequence] (-sequence) perl " -sequence=$value -sformat=fasta" 1 dna 8 seqfile perl 1 required Required section targetrange Specify a Target Range? (-targetrange) 0 perl ($value)? " -targetrange" : "" 2 minprimertm Minimum Primer Tm (deg Celsius) (-minprimertm) 53 perl " -minprimertm=$value" 3 maxprimertm Maximum Primer Tm (deg Celsius) (-maxprimertm) 58 perl " -maxprimertm=$value" 4 advanced Advanced section targetsection Target options targetstart Target start position. (-targetstart) perl $targetrange acd $targetrange acd $sequence.begin perl (defined $value && $value != $vdef)? " -targetstart=$value" : "" 5 targetend Target end position. (-targetend) perl $targetrange acd $targetrange acd $sequence.length perl (defined $value && $value != $vdef)? " -targetend=$value" : "" 6 overlap Minimum overlap of sequences (-overlap) 50 perl (defined $value && $value != $vdef)? " -overlap=$value" : "" 7 primersection Primer options minprimerlen Minimum primer length (-minprimerlen) 18 perl (defined $value && $value != $vdef)? " -minprimerlen=$value" : "" 8 maxprimerlen Maximum primer length (-maxprimerlen) 22 perl (defined $value && $value != $vdef)? " -maxprimerlen=$value" : "" 9 minpmgccont Minimum primer GC fraction (-minpmgccont) .40 perl (defined $value && $value != $vdef)? " -minpmgccont=$value" : "" 10 .30 .70 maxpmgccont Maximum primer GC fraction (-maxpmgccont) .55 perl (defined $value && $value != $vdef)? " -maxpmgccont=$value" : "" 11 .30 .70 productsection Product options minprodlen Minimum product length (-minprodlen) acd @(!$(targetrange)) 100 perl (defined $value && $value != $vdef)? " -minprodlen=$value" : "" 12 maxprodlen Maximum product length (-maxprodlen) acd @(!$(targetrange)) 300 perl (defined $value && $value != $vdef)? " -maxprodlen=$value" : "" 13 minprodgccont Minimum product GC fraction (-minprodgccont) .40 perl (defined $value && $value != $vdef)? " -minprodgccont=$value" : "" 14 .30 .70 maxprodgccont Maximum product GC fraction (-maxprodgccont) .55 perl (defined $value && $value != $vdef)? " -maxprodgccont=$value" : "" 15 .30 .70 saltconc Salt concentration (mM) (-saltconc) 50 perl (defined $value && $value != $vdef)? " -saltconc=$value" : "" 16 1 100 dnaconc DNA concentration (mM) (-dnaconc) 50 perl (defined $value && $value != $vdef)? " -dnaconc=$value" : "" 17 1 100 list Force list-style output (-list) 0 perl ($value)? " -list" : "" 18 output Output section outfile outfile (-outfile) outfile.out perl " -outfile=$value" 19 auto perl " -auto -stdout" 20