4.1f enthalpie, entropy and melting temperature N. Le Novere Nicolas Le Novere (2001), MELTING, computing the melting temperature of nucleic acid duplex. Bioinformatics 17(12), 1226-1227 nucleic:composition melting melting 0 perl "melting -q -v" hybridation_type Hybridisation type (-H) perl " -H$value" 1 dnadna Dna/Dna dnarna Dna/Rna rnarna Rna/Rna nnfile Nearest Neighbor parameters set (-A) perl ($value && $value ne $vdef) ? " -A$value" : "" default default (default) all97a.nn Allawi et al 1997 bre86a.nn Breslauer et al 1986 fre86a.nn Freier et al 1986 san96a.nn SantaLucia et al 1996 sug95a.nn Sugimoto et al 1995 sug96a.nn Sugimoto et al 1996 xia98a.nn Xia et al 1998 1 sequence Sequence string (-S) perl " -S$value" 1 complement_string Complementary sequence (-C) perl $value ? " -C$value" : "" 1 salt_concentratio Salt concentration (0.0 < x < 10.0 M) (-N) perl " -N$value" 1 0 10 nucacid_concentration Nucleic acid concentration in excess (0.0 < x < 0.1 M) (-P) perl $value ? " -P$value" : "" python ("", " -P" + str(value))[value < 0.0] 1 0 0.1 correction_factor Nucleic acid correction factor (-F) perl $value ? " -F$value" : "" 1 salt_correction Salt correction (-K) perl $value ? " -K$value" : "" 1 wet91a Wetmur 1991 san96a SantaLucia et al. 1996 san98a SantaLucia 1998 approx Force approximative temperature computation (-x) perl $value ? " -x" : "" 1 dangling_ends parameters for dangling ends: dnadnade.nn perl ($value)? " -Ddnadnade.nn " : "" 1 mismatches nn parameters for mismatches: dnadnamm.nn perl ($value)? " -Mdnadnamm.nn " : "" 1