loadseq load several sequences into a file edit format loadseq loadseq perl ($seq eq "")? "golden " : "fmtseq -all -p -f9 < " 0 you must either enter a sequence or an accession number perl (! $seq) && (! $query) explanation perl "cp /local/gensoft/doc/loadseq/howto.html .; " -10 howto howto.html seq Sequence to load perl " $seq" 2 This form allows you to progressively load your sequences into a file, one by one. It's purpose is to help users who do not have a concatenate utility on their computer to build a multiple sequence file. The sequence is automatically converted in FASTA format. Load your first sequence, and wait for the output. You will be able to choose loadseq in the menu to go on with additional sequences. You may also provide an accession number or entry name, which will be fetched and loaded in the sequences file (advanced form). You may also load more than one sequence at a time. name You may give a name to your sequence perl ($seq ne "" && $name ne "")? "; mv load_result tmp; sed \\"s/>,*/> $name/\\" tmp > load_result" : "" 30 If you load not more than one sequence... no message perl ($name eq "" && $seq ne "seq.data" && ($name=$seq) && 0) loadresult perl " > load_result" 20 efetch_params Efetch parameters perl ! $seq db Database perl " $db:$query | flat2fasta - " genbank 2 uniprot genpept genbank embl nrl3d prosite perl ! $seq query query (Entry name or AC) perl "" 3 perl ! $seq ac Search with Accession number (except nrl3d) perl ($value)? " -a":"" 0 1 No Accesion number for Nrl3d perl $ac && $db eq "nrl3d" perl ! $seq outfile perl "" "loadseq.out" seqsfile perl 1 other_options Other parameters seqfile Already loaded sequences file perl ($value)? "; cat $seqfile load_result" : "; cat load_result" 40 You don't need to enter this parameter, normally. You should enter your sequences above (Sequence to load). This is the intermediate file containing already loaded sequences. seqsfile perl 1