]> Protein identification by mass spectrometry (EMBOSS) protein:composition http://bioweb.pasteur.fr/docs/EMBOSS/emowse.html emowse &emboss_init; input Input section sequence sequence -- protein [sequences] (-sequence) perl " -sequence=$value -sformat=fasta" 1 protein 8 seqsfile perl 1 infile Name of molecular weight data file (-infile) perl " -infile=$value" 2 required Required section weight Whole sequence molwt (-weight) 0 perl " -weight=$value" 3 advanced Advanced section enzyme Enzyme or reagent -- Enzymes and reagents (-enzyme) 1 Trypsin 2 Lys-C 3 Arg-C 4 Asp-N 5 V8-bicarb 6 V8-phosph 7 Chymotrypsin 8 CNBr 1 perl " -enzyme=$value" 4 aadata Amino acid data file (-aadata) Eamino.dat perl ($value && $value ne $vdef)? " -aadata=$value" : "" 5 Molecular weight data for amino acids pcrange Allowed whole sequence weight variability (-pcrange) 25 perl (defined $value && $value != $vdef)? " -pcrange=$value" : "" 6 75 frequencies Frequencies file (-frequencies) Efreqs.dat perl ($value && $value ne $vdef)? " -frequencies=$value" : "" 7 tolerance Tolerance (-tolerance) 0.1 perl (defined $value && $value != $vdef)? " -tolerance=$value" : "" 8 0.1 1.0 partials Partials factor (-partials) 0.4 perl (defined $value && $value != $vdef)? " -partials=$value" : "" 9 0.1 1.0 output Output section outfile outfile (-outfile) outfile.out perl " -outfile=$value" 10 auto perl " -auto -stdout" 11