ID   DNJB1_HUMAN    STANDARD;      PRT;   339 AA.
AC   P25685;
DT   01-MAY-1992, integrated into UniProtKB/Swiss-Prot.
DT   16-AUG-2004, sequence version 3.
DT   25-JUL-2006, entry version 66.
DE   DnaJ homolog subfamily B member 1 (Heat shock 40 kDa protein 1) (Heat
DE   shock protein 40) (HSP40) (DnaJ protein homolog 1) (HDJ-1).
GN   Name=DNAJB1; Synonyms=DNAJ1, HDJ1, HSPF1;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
OC   Catarrhini; Hominidae; Homo.
OX   NCBI_TaxID=9606;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Placenta;
RX   MEDLINE=92093635; PubMed=1754405;
RA   Raabe T., Manley J.L.;
RT   "A human homologue of the Escherichia coli DnaJ heat-shock protein.";
RL   Nucleic Acids Res. 19:6645-6645(1991).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA], AND PROTEIN SEQUENCE OF 1-47.
RC   TISSUE=Placenta;
RX   MEDLINE=94071949; PubMed=8250930;
RA   Ohtsuka K.;
RT   "Cloning of a cDNA for heat-shock protein hsp40, a human homologue of
RT   bacterial DnaJ.";
RL   Biochem. Biophys. Res. Commun. 197:235-240(1993).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   TISSUE=Placenta;
RX   MEDLINE=97131529; PubMed=8975727; DOI=10.1006/geno.1996.0653;
RA   Hata M., Okumura K., Seto M., Ohtsuka K.;
RT   "Genomic cloning of a human heat shock protein 40 (Hsp40) gene (HSPF1)
RT   and its chromosomal localization to 19p13.2.";
RL   Genomics 38:446-449(1996).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Brain, and Lung;
RX   MEDLINE=22388257; PubMed=12477932; DOI=10.1073/pnas.242603899;
RA   Strausberg R.L., Feingold E.A., Grouse L.H., Derge J.G.,
RA   Klausner R.D., Collins F.S., Wagner L., Shenmen C.M., Schuler G.D.,
RA   Altschul S.F., Zeeberg B., Buetow K.H., Schaefer C.F., Bhat N.K.,
RA   Hopkins R.F., Jordan H., Moore T., Max S.I., Wang J., Hsieh F.,
RA   Diatchenko L., Marusina K., Farmer A.A., Rubin G.M., Hong L.,
RA   Stapleton M., Soares M.B., Bonaldo M.F., Casavant T.L., Scheetz T.E.,
RA   Brownstein M.J., Usdin T.B., Toshiyuki S., Carninci P., Prange C.,
RA   Raha S.S., Loquellano N.A., Peters G.J., Abramson R.D., Mullahy S.J.,
RA   Bosak S.A., McEwan P.J., McKernan K.J., Malek J.A., Gunaratne P.H.,
RA   Richards S., Worley K.C., Hale S., Garcia A.M., Gay L.J., Hulyk S.W.,
RA   Villalon D.K., Muzny D.M., Sodergren E.J., Lu X., Gibbs R.A.,
RA   Fahey J., Helton E., Ketteman M., Madan A., Rodrigues S., Sanchez A.,
RA   Whiting M., Madan A., Young A.C., Shevchenko Y., Bouffard G.G.,
RA   Blakesley R.W., Touchman J.W., Green E.D., Dickson M.C.,
RA   Rodriguez A.C., Grimwood J., Schmutz J., Myers R.M.,
RA   Butterfield Y.S.N., Krzywinski M.I., Skalska U., Smailus D.E.,
RA   Schnerch A., Schein J.E., Jones S.J.M., Marra M.A.;
RT   "Generation and initial analysis of more than 15,000 full-length human
RT   and mouse cDNA sequences.";
RL   Proc. Natl. Acad. Sci. U.S.A. 99:16899-16903(2002).
RN   [5]
RP   PROTEIN SEQUENCE OF 1-48, INDUCTION, AND SUBCELLULAR LOCATION.
RX   MEDLINE=92266438; PubMed=1586970;
RA   Hattori H., Liu Y.-C., Tohnai I., Ueda M., Kaneda T., Kobayashi T.,
RA   Tanabe K., Ohtsuka K.;
RT   "Intracellular localization and partial amino acid sequence of a
RT   stress-inducible 40-kDa protein in HeLa cells.";
RL   Cell Struct. Funct. 17:77-86(1992).
RN   [6]
RP   INTERACTION WITH DNAJC3.
RX   MEDLINE=99121125; PubMed=9920933; DOI=10.1074/jbc.274.6.3797;
RA   Melville M.W., Tan S.-L., Wambach M., Song J., Morimoto R.I.,
RA   Katze M.G.;
RT   "The cellular inhibitor of the PKR protein kinase, P58(IPK), is an
RT   influenza virus-activated co-chaperone that modulates heat shock
RT   protein 70 activity.";
RL   J. Biol. Chem. 274:3797-3803(1999).
RN   [7]
RP   STRUCTURE BY NMR OF 1-75.
RX   MEDLINE=96291433; PubMed=8764402; DOI=10.1006/jmbi.1996.0394;
RA   Qian Y.Q., Patel D., Hartl F.-U., McColl D.J.;
RT   "Nuclear magnetic resonance solution structure of the human Hsp40
RT   (HDJ-1) J-domain.";
RL   J. Mol. Biol. 260:224-235(1996).
CC   -!- FUNCTION: Interacts with HSP70 and can stimulate its ATPase
CC       activity. Stimulates the association between HSC70 and HIP.
CC   -!- SUBUNIT: Interacts with DNAJC3.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm. Translocates rapidly from the
CC       cytoplasm to the nuclei, and especially to the nucleoli, upon heat
CC       shock.
CC   -!- INDUCTION: By heat shock.
CC   -!- SIMILARITY: Contains 1 J domain.
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DR   EMBL; X62421; CAA44287.1; -; mRNA.
DR   EMBL; D49547; BAA08495.1; -; mRNA.
DR   EMBL; D85429; BAA12819.1; -; Genomic_DNA.
DR   EMBL; BC002352; AAH02352.1; -; mRNA.
DR   EMBL; BC019827; AAH19827.1; -; mRNA.
DR   PIR; JN0912; JN0912.
DR   PIR; S20062; S20062.
DR   UniGene; Hs.515210; -.
DR   PDB; 1HDJ; NMR; @=1-75.
DR   Ensembl; ENSG00000132002; Homo sapiens.
DR   H-InvDB; HIX0014838; -.
DR   HGNC; HGNC:5270; DNAJB1.
DR   MIM; 604572; gene.
DR   LinkHub; P25685; -.
DR   RZPD-ProtExp; RZPDo834C0320; -.
DR   RZPD-ProtExp; RZPDo834C0428; -.
DR   RZPD-ProtExp; T0399; -.
DR   GO; GO:0006986; P:response to unfolded protein; TAS.
DR   InterPro; IPR002939; DnaJ_C.
DR   InterPro; IPR001623; DnaJ_N.
DR   InterPro; IPR008971; HSP_Dna_pep_bd.
DR   InterPro; IPR003095; Hsp_DnaJ.
DR   Pfam; PF00226; DnaJ; 1.
DR   Pfam; PF01556; DnaJ_C; 1.
DR   PRINTS; PR00625; DNAJPROTEIN.
DR   SMART; SM00271; DnaJ; 1.
DR   PROSITE; PS00636; DNAJ_1; 1.
DR   PROSITE; PS50076; DNAJ_2; 1.
KW   3D-structure; Chaperone; Direct protein sequencing; Heat shock;
KW   Nuclear protein.
FT   INIT_MET      0      0
FT   CHAIN         1    339       DnaJ homolog subfamily B member 1.
FT                                /FTId=PRO_0000071016.
FT   DOMAIN        1     69       J.
FT   CONFLICT     10     10       L -> Q (in Ref. 1).
FT   CONFLICT     12     27       RGASDEEIKRAYRRQA -> AALGRGDQAGLPPPG (in
FT                                Ref. 1).
FT   CONFLICT     67     67       G -> L (in Ref. 1).
FT   CONFLICT     80    135       SGGGANGTSFSYTFHGDPHAMFAEFFGGRNPFDTFFGQRNG
FT                                EEGMDIDDPFSGFPM -> TAEEPMVPLSATHSMETLMPCL
FT                                LSSSVAEIPLTPFLGSGTGRKAWTLMTHSLASLW (in
FT                                Ref. 1).
FT   CONFLICT    149    149       R -> C (in Ref. 1).
FT   CONFLICT    182    182       M -> T (in Ref. 1).
FT   CONFLICT    319    319       V -> A (in Ref. 1).
FT   STRAND        4      4
FT   HELIX         5      8
FT   TURN          9      9
FT   TURN         12     13
FT   HELIX        16     28
FT   TURN         29     30
FT   TURN         32     34
FT   TURN         38     39
FT   HELIX        40     53
FT   TURN         54     55
FT   HELIX        57     65
FT   TURN         66     66
FT   HELIX        68     70
FT   STRAND       73     74
SQ   SEQUENCE   339 AA;  37913 MW;  7985B4125FB4B12E CRC64;
     GKDYYQTLGL ARGASDEEIK RAYRRQALRY HPDKNKEPGA EEKFKEIAEA YDVLSDPRKR
     EIFDRYGEEG LKGSGPSGGS GGGANGTSFS YTFHGDPHAM FAEFFGGRNP FDTFFGQRNG
     EEGMDIDDPF SGFPMGMGGF TNVNFGRSRS AQEPARKKQD PPVTHDLRVS LEEIYSGCTK
     KMKISHKRLN PDGKSIRNED KILTIEVKKG WKEGTKITFP KEGDQTSNNI PADIVFVLKD
     KPHNIFKRDG SDVIYPARIS LREALCGCTV NVPTLDGRTI PVVFKDVIRP GMRRKVPGEG
     LPLPKTPEKR GDLIIEFEVI FPERIPQTSR TVLEQVLPI
//