This test covers the ViPR use case where a non-default protein matrix is used with bootstrapping: tool=RAXMLHPC2_TGB vparam.runtime_=168 (we use cipres run wall time as suggested by you) vparam.bootstrap_seed_=1 (we use bootstrap_seed to indicates bootstrapping, this will be 0 for non-bootstrapping) vparam.bootstop_= vparam.prot_sub_model_=PROTGAMMA vparam.prot_matrix_spec_= Generates this command line: raxmlHPC-HYBRID -n result -s infile.txt -x 12345 -m PROTGAMMALG4M -N 50 -p 12345 -f a