This is test covers the ViPR use case where bootstrapping is used, and DNA data: tool=RAXMLHPC2_TGB vparam.runtime_=168 (we use cipres run wall time as suggested by you) vparam.bootstrap_seed_=1 (we use bootstrap_seed to indicates bootstrapping) vparam.bootstop_=50 vparam.dna_gtrcat_=GTRGAMMA for dna sequences; for protein sequences, we use the following two: Generates this command line: 'raxmlHPC-HYBRID -n result -s infile.txt -x 12345 -N 50 -p 12345 -f a -m GTRGAMMA '