This test suite covers the ViPR use case where no bootstrapping is used, with DNA data: tool=RAXMLHPC2_TGB vparam.runtime_=168 (we use cipres run wall time as suggested by you) vparam.bootstrap_seed_=0 (this will be 0 for non-bootstrapping) vparam.dna_gtrcat_=GTRGAMMA for dna sequences At present we must set vparam.bootstrap_seed_=0 Generates the command line: raxmlHPC-PTHREADS -n result -s infile.txt -p 12345 -m GTRGAMMA '