]> X.X A Bayesian approach to refinement of (multiple) 3D reconstructions or 2D class averages in electron cryo-microscopy (cryo-EM). S. H. W. Scheres Scheres, S. H. W. (2012) RELION: Implementation of a Bayesian approach to cryo-EM structure determination. Journal of Structural Biology 180, 519-530 Structural Biology http://www2.mrc-lmb.cam.ac.uk/relion/index.php/Main_Page relion_xsede relion_invoke perl "-v relion_refine_mpi" 0 relion_commandline perl "--ctf --flatten_solvent --zero_mask --oversampling 1 --healpix_order 2 --auto_local_healpix_order 4 --low_resol_join_halves 40 --norm --scale  --j 1 --memory_per_thread 4" 1 infile Images File (FASTA format) input.images perl "-i input.images" 90 relion_scheduler scheduler.conf perl "ntasks-per-node=15\\n" . "node_exclusive=1\\n" . "nodes=10\\n" 1 all_outputfiles * runtime 1 scheduler.conf Maximum Hours to Run (click here for help setting this correctly) 12 Estimate the maximum time your job will need to run (up to 168 hrs). Your job will be killed if it doesn't finish within the time you specify, however jobs with shorter maximum run times are often scheduled sooner than longer jobs. Maximum Hours to Run must be between 0.1 - 168.0. perl $runtime < 0.1 || $runtime > 168.0 perl "runhours=$value\\n" outfile_root 2 Supply the base name for the output file perl "-o $value" This value determines the name of output files. particle_dia 3 Diameter of particle (Angstroms) perl "—-particle_diameter $value" Please enter a value for the particle diameter (in Angstroms) perl !$particle_dia pixel_size 4 Pixel size of data (A/pix) perl "—angpix $value" 1 initial_filter 5 Initial filter for 3D volume perl "—-ini_high $value" Please enter a value for the Iniital filter for 3D volume perl !$initial_filter offset_range 6 Pixel search range perl "—-offset_range $value" 5 offset_stepsz 7 Pixel search range step size perl "—-offset_step $value" 1 symmetry_sample 8 Symmetry of sample perl "—sym $value" C1